Which of the following substances would NOT typically be tested for under a DOT drug test?

In the context of DOT drug testing, nicotine is not included in the list of substances that are typically screened for. The Department of Transportation mandates testing for specific illicit drugs and their metabolites, including cocaine, marijuana, and other controlled substances such as amphetamines and opiates. Alcohol is also an essential component of testing under DOT regulations due to its potential impact on safety.

Nicotine, however, does not fall under the category of illegal substances or safety-sensitive pre-employment testing criteria outlined by the DOT. While it is a substance that can impact health and well-being, it is legal and is not considered a substance leading to impaired performance in the same way that drugs like cocaine and marijuana can affect an individual's ability to operate vehicles or perform safety-sensitive functions. Therefore, the absence of nicotine from the drug testing panel means it would not typically be tested under DOT guidelines.