What is the minimum amount of urine required in the secondary specimen bottle for DOT split specimen collections?

In the context of DOT split specimen collections, the minimum amount of urine required in the secondary specimen bottle is established to ensure that adequate sample volume is available for additional testing if necessary. The correct response reflects the requirement that at least 15 mL of urine be collected in the secondary specimen container.

This volume is significant because it allows for confirmatory testing, which is essential if the initial screening tests are positive or inconclusive. Having a sufficient amount of urine ensures that laboratories can perform all necessary analyses, including further validation of results, while also providing the opportunity for the donor to verify the findings if they choose to contest a positive result. Thus, this specific volume supports the integrity of the testing process and the rights of the individuals undergoing testing, making it crucial for compliance with DOT regulations.